Bark would almost certainly contain the fungus, so using this would hit the Ascomyta via its DNA as well as via the DNA of the tree.
A healthy twig may be unlikely to be infected, but will still treat the tree. The same should be true for a leaf.
I would run frequencies for it non-stop for at least a week, then evaluate.
For more details, please check the link:
http://www.spooky2.com/forums/viewtopic.php?f=18&t=5106
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